Journal Details


Volume Number:  4
Jurnal Name:  Efflatounia

Authors: 
               Moataza A. Dorrah
               Tahany H. Ayaad

Title:
LOCALIZATION, PURIFICATION, AND N-TERMINAL AMINO ACID SEQUENCE OF PROPHENOLOXIDASE OF PARASARCOPHAGA SURCOUFI PREPUPAE (DIPTERA: SARCOPHAGIDAE)

Keywords: 
Prophenoloxidase, localization, purification, N-terminal amino acid sequence, Parasarcophaga surcoufi

Abstract:
Prophenoloxidase (PPO) is a key enzyme associated with both melanin biosynthesis and sclerotization in insects. In the present work, PPO was purified from prepupae of the fleshfly Parasarcophaga surcoufi by employing ammonium sulphate precipitation and DEAE-cellulose column chromatography. SDS-PAGE revealed that the molecular weight of the purified PPO is 90 KDa. N-terminal amino acid-sequence of the purified enzyme subunit was determined; it was found to be homologous to arthropod hemocyanin, with 62% identity to that of Limulus polyphemus.

Paper Pdf. File:
Paper Pdf. File
http://www.efflatounia.com/files/04-01.pdf