Journal Details


Volume Number:  4
Jurnal Name:  Efflatounia

Authors: 
               Moataza A. Dorrah

Title:
EFFECT OF SOYBEAN TRYPSIN INHIBITOR ON DIGESTIVE PROTEASES AND GROWTH OF LARVAL SPODOPTERA LITTORALIS (BOISD.)

Keywords: 
Proteases, soybean trypsin inhibitor, growth, development, Spodoptera littoralis .

Abstract:
Two serine proteases were identified in midgut of 4th instar of Spodoptera littoralis. A trypsin-like one (BApNA-hydrolyzing) (TRYP) with a pH optimum at 11.0, and a chymotrypsin esterase-like enzyme (BTEE-hydrolyzing) (CHYMO) with optimum activity at pH 10.0. Serine protease activities were significantly (p < 0.001) inhibited in vitro by soybean trypsin inhibitor (SBI). The incorporation of different concentrations of SBI into an artificial diet, consumed by the larvae, causes variable in vivo inhibitory effects. These effects were more prominent in the fourth instar; however, in the fifth instar the proteases activity recoverd again. The ingestion of dietary SBI also reduces the weight gain and survival of the larvae.

Paper Pdf. File:
Paper Pdf. File
http://www.efflatounia.com/files/04-03.pdf