Journal Details


Volume Number:  9
Jurnal Name:  Efflatounia

Authors: 
               Moataza A. Dorrah

Title:
PHENOLOXIDASE AND LYSOZYME ACTIVITY IN NAIVE AND IMMUNE-RESPONSIVE LARVAL PARASARCOPHAGA HERTIPES (DIPTERA: SARCOPHAGIDAE)

Keywords: 
Lysozymes; phenoloxidase; activity; immune challenge; midgut; hemolymph; Cyclorrhapha; Diptera; larvae.

Abstract:
Hemolymph phenoloxidase (PO) and prophenoloxidase (proPO), and lysozyme, as well as midgut lysozyme in larval Parasarcophaga hertipes have been identified and partially characterized. PO activity is optimum at pH 6.0 and has a Km of 0.43 mM for dopamine as a substrate. Midgut and hemolymph lysozymes have optimum pHs at pH 4.5 and 6.0 and Km values of 20.0 and 0.37 mM, respectively, for the Micrococcus lysodeikticus cell wall. In naive larva, the hemolymph PO: proPO was 1:5. After immune challenge, the two enzyme forms increased after 1 h and reached a maximum (~ 4 times the naive larva value) and PO: proPO became 1:1 after 24 h post challenging. The hemolymph lysozyme also increased after 1 h, and reached a maximum (~ 3 times the naive larva value) 24 h post challenging. The midgut lysozyme is a digestive enzyme, and did not increase on immune challenging. Spiking of the larva causes elevation of phenoloxidase forms to 2-3 times the naive level, but not the lysozyme. The presented results are discussed.

Paper Pdf. File:
Paper Pdf. File
http://www.efflatounia.com/files/09-02.pdf