Journal Details


Volume Number:  9
Jurnal Name:  Efflatounia

Authors: 
               Mohamed Elmogy
               Makio Takeda

Title:
BIOCHEMICAL AND CELLULAR CHANGES DURING THE PROGRAMMED CELL DEATH IN THE FAT BODY CELLS OF THE SILKWORM BOMBYX MORI

Keywords: 
Programmed cell death; apoptosis; autophagy; biochemical changes; remodeling; immunolocalization; cellular changes; Bombyx .

Abstract:
The fat body cells of Bombyx mori undergo remodeling process during larval-pupal metamorphosis. Part of this remodeling is the massive destruction of these cells by PCD. The celluar observations from the histochemically HE stained fat body sections during larval-pupal metamorphosis revealed a definite apoptotic morphological features whose sequence started from G1. Different classes of apoptotic nuclei could be observed as an emitted colour gradient, which reflects the degree of chromatin condensation, as revealed by the histochemicaly double stained [AO/HO] sections. The proliferated cell nuclei could be detected and localized by the mitotic marker phosphohistone H3 antibody, as revealed by the immunohistochemical and Western blot analysis. The obtained results show that the massive destruction of Bombyx fat body cells by PCD during larval pupal metamorphosis is accompained with a simultaneous proliferation of the adult progenitor cells. The studied biochemical changes revealed genomic DNA fragmentation at G3 followed by DNA ladder formation at P, which are considered as biochemical markers of apoptosis. Caspase-3 activity is required for DNA degradation as revealed by Western blot analysis. Autophagy features are also exhibited during the prepupal period. Levels of one of the lysosomal marker enzymes, acid phosphatase, are detected to be up regulated by the hemolymph ecdysteroid titer and maximized at late prepupal period (G3). The ubiquitin-proteasome mechanism is also involved during the degradation of fat body cells during the late prepupal and pupal periods (G3 and P) as revealed by Western blot analysis. Apoptosis and autophagic cell death interact with each other during the degradation process of the fat body cells in Bombyx mori and this interaction occurs at a late phase of cell death (G3).

Paper Pdf. File:
Paper Pdf. File
http://www.efflatounia.com/files/09-03.pdf