Journal Details


Volume Number:  15
Jurnal Name:  Efflatounia

Authors: 
               Moataza A. Dorrah
               Eman A. Abdelfattah

Title:
DOPA AND FERROUS IRON INCREASE DNA STRAND-BREAKS IN THE DESERT LOCUST SCHISTOCERCA GREGARIA (ORTHOPTERA: ACRIDADE)

Keywords: 
Oxidative stress; DNA damage; DOPA; Fe2+; Schistocerca

Abstract:
Redox-cycling phenolics and redox-active metals are able to generate active oxygen species and oxidative stress during their metabolism. Under these conditions, oxidative damage to cell macromolecules including DNA may occur. Interahemocoelic injection of the redox-cycling compound DOPA or the redox-active Fe2+ (FeSO4) into the 5th instar Schitocerca gregaria caused oxidative damage to DNA in the form of strand-breaks. The strand-breaks were measured by alkaline single-cell gel electrophoresis (comet) assay as tail moment values (tail length ×% DNA in tail) in addition to % severed-cells. Post injection of DOPA or Fe2+ (FeSO4), the values of the tail moment and % severed-cells increased several times than those of the control in both thoracic muscles and midgut cells. The obtained data were discussed emphasizing autoxidation and redox-cycling properties of DOPA and its pro-oxidant and antioxidant behaviors, as well as participation of the redox-active metal iron and possible formation of iron-oxygen complexes in production of reactive oxygen species and oxidative damage.

Paper Pdf. File:
Paper Pdf. File
http://www.efflatounia.com/files/15 37-1.pdf